Since mid-September, when she gave birth to a litter of seven puppies, the mixed-breed stray pooch has been in the care of students at Zhengzhou University in Henan province.
The video, which was shot on Monday, shows the tearful mother kissing her brown pup goodbye before sending it off with its new owner.
The female owner was spotted carrying the young pup in her arms to her scooter, as the concerned mother trailed after.
The mother dog came to a halt next to the owner’s scooter, head down, tail down, and ears drooping, as if pleading to see her pup again.